![]() ![]() Optimal substrate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Primer Extension with Klenow fragment īiotin-14-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. Nick Translation with DNAse I/ DNA Polymerase I & in-house data Molecular Weight: 905.78 g/mol (free acid)įorm: filtered solution (30 kDa) in 10 mM Tris-HCl Molecular Formula: C 31H 54N 7O 16P 3S (free acid) Shelf Life: 12 months after date of delivery ![]() Short term exposure (up to 1 week cumulative) to ambient temperature possible. RNA Labeling Selector (Kits & Nucleotides).DNA Labeling Selector (Kits & Nucleotides).Privacy Policy for Jena Bioscience's Facebook Page.Poly (A) carrier RNA-based RNA purification.3'-End RNA Labeling (T4 RNA Ligase 1-based).Random RNA Labeling ( in vitro Transcription-based).Click Chemistry-based RNA/cRNA Labeling.Click Chemistry-based DNA/cDNA Labeling.Quantifoil® Holey Carbon Films with 2 nm Continuous Carbon.Mercurated and Selenium-containing Nucleotides.JBS Tungsten Cluster Derivatization Kits.Cryo and Room Temperature Crystallography.in Posttranslational Modification Analysis Immobilized Nucleotides for Affinity Chromatography.on Proteins/Enzymes - Sulfonyl Fluoride Probes Cancer and Proliferation Marker Nucleosides. ![]()
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